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Primer sequences for quantitative real‐time PCR analysis
| Gene | Sense primer sequence (5′–3′) | Antisense primer sequence (5′–3′) |
|---|---|---|
| GAPDH | CCTCCTGCACCACCAACTGCTT | GAGGGGCCATCCACAGTCTTCT |
| MYF5 | ATGGACGTGATGGATGGCTG | GCGGCACAAACTCGTCCCCAA |
| MYOD | GGTCCCTCGCGCCCAAAAGAT | CAGTTCTCCCGCCTCTCCTAC |
| MYOG | CAGTGCACTGGAGTTCAGCG | TTCATCTGGGAAGGCCACAGA |
GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; MYF5, myogenic factor‐5; MYOD, myogenic differentiation factor; MYOG, myogenin.
