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. 2018 Sep 5;8(1):22–34. doi: 10.1002/sctm.18-0085

Figure 4.

Figure 4

Characterization of MSC culture on MgHA/Col scaffolds. (A) Confocal analysis of LVhsRL‐transduced Rankl −/− MSC‐seeded scaffolds at different time points; blue color corresponds to collagen fibers, green to MSCs. Scale bar: 200 μm (B) Assessment of WT, mock‐transduced and LVhsRL‐transduced Rankl −/− MSC proliferation in 2D versus 3D culture overtime by MTT assay. Cell growth is expressed as fold increase compared to time 0. Statistics is referred to comparison of 2D versus 3D for each group. Cell growth fold increase data ± SEM are reported in Supporting Information Table 1. (C) Evaluation of hsRL concentration in the supernatant of LVhsRL‐transduced Rankl −/− MSCs in 2D versus 3D culture by ELISA assay. * p < .05; ** p < .01.