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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Exp Physiol. 2018 Nov 12;104(1):126–135. doi: 10.1113/EP087278

Figure 4.

Figure 4

ATP synthase specific activity in skeletal muscle of lean and obese subjects. ATP synthase activity was measured as the rate of ATP hydrolysis to ADP, ultimately coupled to the oxidation of NADH to NAD+ and monitored as the change in absorbance at 340 nm (i.e., nm/minute/amount of sample loaded into the well). The quantity of the enzyme was measured using an ATP synthase specific antibody conjugated with alkaline phosphatase, and by monitoring the change in absorbance at 405 (i.e., nm/minute/amount of sample loaded into the well). ATP synthase specific activity was calculated as the ratio of the absorbance at 340 nm to that at 405 nm and it is reported as arbitrary units. Values are mean ± SD. *P < 0.05 versus lean (unpaired two-sided t test).