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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Anat Rec (Hoboken). 2018 Oct 10;302(1):125–135. doi: 10.1002/ar.23916

FIGURE 4: Cdh5(PAC)-CreERT2;Rosa26mTmG mice exhibit robust recombination efficiency and specificity to vascular endothelial cells.

FIGURE 4:

A-D: P7 heart valves exhibit robust GFP expression in valve endothelial cells (VECs) after postnatal tamoxifen induction. E-G: GFP expression is also observed in vascular endothelial cells from other regions of the heart and lungs. H-K: 2 month-old heart valves continue to express GFP in VECs after postnatal tamoxifen induction. L-N: Similarly, GFP expression is also observed in endothelial cells from other regions of the heart and lungs. O: Schematic of experiment. Tamoxifen was administered via intragastric injections once per day from post-natal day 0 (P0) to P2. Hearts were harvested at either P7 or 2 months-of-age, sectioned, and analyzed for GFP expression. Scale Bar = 50μm.