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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Anat Rec (Hoboken). 2018 Oct 10;302(1):125–135. doi: 10.1002/ar.23916

FIGURE 6: Hematopoietic cells do not arise from valve endothelial cells in adult Marfan syndrome mice.

FIGURE 6:

A-B: Fluorescent detection of endothelial cells (CD31+, GFP) and hematopoietic cells (CD45+) in 2 month-old Fbn1+/+ mitral valves. CD45+ cells (white) are a distinct population from VECs (green) (Panels A’-A’’’ and B’-B’’’). C-D: 2 month-old Fbn1C1039G/+ Marfan syndrome mitral valves are thickened and exhibit more CD45+ cells versus control. CD45+ cells do not express the Cdh5-CreERT2-derived GFP lineage marker (Panels C’-C’’’ and D’-D’’’). E: qPCR analysis using RNA isolated from Fbn1C1039G/+ mitral valves demonstrates increased mRNA expression of Ccl2 at 2 and 4 months-of-age, but not at 1 month, versus Fbn1+/+ controls (n=9 per genotype per time-point). Mann-Whitney U Test was used with statistical significance defined as *p<0.05. Data are reported with error bars indicating SD. Scale Bar = 50μm.