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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Anat Rec (Hoboken). 2018 Oct 5;302(1):136–145. doi: 10.1002/ar.23909

Figure 5. 3D AMIRA reconstruction of the arrangement of the pulmonary veins in a normal embryonic mouse heart at 14.5ED.

Figure 5.

The reconstruction in panel A was prepared using serial sections of a Tie2-cre;ROSA26mT/mG mouse embryo at 14.5ED. All sections were stained with a combination of antibodies recognizing both sarcomeric actin and smooth muscle actin (red in B), a combination of antibodies useful for recognizing cardiomyocytes and smooth muscle cells. Endothelial and endothelial-derived cells in the Tie2-cre;ROSA26mT/mG mouse express eGFP (green in B). C shows the 3D reconstruction “sliced” at the level of the tissue section shown in B. The pulmonary veins in panel A are pseudocolored in yellow, the left sinus horn in blue. AS=atrial septum; CPV=common pulmonary vein; LA=left atrium; LPV=left pulmonary veins; LSH=left sinus horn; LV=left ventricle; RA=right atrium; RPV=right pulmonary vein; RV=right ventricle