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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: DNA Repair (Amst). 2018 Nov 13;73:64–70. doi: 10.1016/j.dnarep.2018.11.003

Fig. 2.

Fig. 2.

GFP reporter assays for HR in p12KO cells. Cells were analyzed for HR activity using the DR-GFP based reporter assay (Material and Methods). Data show the HR activity expressed as % GFP positive cells (n=3). Statistical analysis was performed using Graphpad Prism software. (A) H1299 and H1299-p12KO cells. (B) H1299, H1299-p12KO and H1299-p12KO cells into which Flag-tagged p12 was stably expressed. (C) H1299, H1299-p12KO and H1299-p12KO+Flag-p12 cells were Western blotted for p12, Flag-p12 and the p125 subunit of Pol δ. B-actin was used as a loading control.