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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: J Comp Neurol. 2018 Nov 11;527(1):282–296. doi: 10.1002/cne.24507

Figure 7: Formation of AOS midbrain circuitry requires Sema6A.

Figure 7:

(a) Schematic of retrograde labeling of MTN neurons by injection of CTB into the NOT. (b-e) Retrograde labeling of MTN-NOT projections neurons in control (b, c) and Sema6A−/− (d, e) animals with CTB-555 reveals a loss of MTN-NOT connection in the Sema6A mutants (n=3 animals per genotype). CTB-488 injected into the contralateral eye defines the extent of RGC MTN innervation (green, c, e). (f-m) Examination of MTN neurons labeled in the HB9-GFP line reveals that at P4 (f-i) and P30 (j-m), MTN neurons are reduced and GFP+ processes extending between the MTNv and MTNd (yellow arrows) and from the MTNv into the sf-AOT (cyan arrows) are absent in Sema6A mutants. Residual CTB-555 from the eye co-localizes only with the remaining HB9-GFP+ MTN neurons (n=3 animals per genotype). Scale bars: f-q, 50 microns.