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. 2018 Nov 7;2(1):1–11. doi: 10.1093/abt/tby011

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© The Author(s) 2018. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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NGS analysis of the shark V_NAR library. (A) Flow chart of the criteria used to categorize the V_NAR types for 1.2 million validated full-length shark V_NAR sequences. (B) Pie chart of the percentage of repeated sequences. Numbers represent the times each unique sequences are repeated in NGS results. (C) Pie chart of the percentage of four types of V_NARs and other type of V_NARs. (D) Pie chart of the percentage of classical Type I V_NARs with six cysteines and the other non-canonical Type I V_NARs. (E) Pie chart of the percentage of classical Type II V_NARs with four cysteines and the other non-canonical Type II V_NARs.