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. 2019 Jan 1;6:1. doi: 10.1038/s41438-018-0066-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The Venn diagrams omit the numbers of differentially expressed genes in multiple comparisons (overlapping areas) and just report the number of up- (left) and down-regulated (right) genes specific to each comparison indicated at the vertexes. The number of genes that maintained the same differential transcription pattern in curly vs smooth cultivars (core-DEGs) is bolded. b Scheme of sesquiterpenoid and triterpenoid biosynthesis pathway in endive. Rectangles report the enzyme codes; and those assigned to endive contigs are in green (see Table 5). GAS/GAO/COS gene module acts in the costunolide synthesis branch. c Expression profiles of eight STP unigenes achieved by RNA-seq and qPCR (gray and black histograms, respectively). The unigene names in bold belong to the core-DEGs group. The last panel reports RNA-seq/qRT-PCR correlation analysis (bottom right) expressed as log2 fold change of curly vs smooth genotypes (six independent comparisons per gene); significant positive correlation occurred between the expression fold changes measured by the two methods (R² = 0.89; P < 0.001)