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. 2018 Dec 12;3(12):17164–17172. doi: 10.1021/acsomega.8b02918

Figure 2.

Figure 2

(a) Representative fluorescence images showing the concentration-dependent uptake of green 5%-QD–SVS-1 conjugates. The cells were co-incubated with various concentrations of QD–SVS-1 and Texas-Red-transferrin for 1 h at 37 °C. Cells incubated with QDs at 75 nM show no uptake. (b) Fluorescence images of HeLa cells incubated with 5%-QD–SVS-1 (50 nM) for 15, 30, 60, and 90 min at 37 °C; co-labeling of the endosomes with Texas-Red-transferrin (red, 60 min incubation) was limited to cultures incubated with the conjugates for 60 and 90 min. Flow cytometry analysis of conjugate internalization for: (c) green 5%-QD–SVS-1 by CHO cells (light gray bars) and HeLa cells (dark gray bars); (d) green 5%-QD–SVS-1 (light gray bars) and green 10% QD–SVS-1 (gray bars) by HeLa cells; (e) green-(light gray bars), yellow-(gray bars) and red-(dark gray bars) emitting 5%-QD–SVS-1 conjugates by HeLa cells. All of the flow cytometry measurements were carried out using incubation for 1 h at 37 °C. Scale bar ∼ 10 μm.