Figure 4.

The OCH1 silencing affects the content in N-linked and O-linked glycans at the cell wall of Sporothrix schenckii.

Notes: Yeast-like cells were incubated with either endoglycosidase H or β-eliminated, to trim N-linked or O-linked glycans, respectively, and the sugar content was quantified as described in the “Materials and methods” section. Data are represented as mean ± SD of three independent experiments performed in duplicates. *P<0.05 when compared to WT cells. The strains used are 1099-18 ATCC MYA 4821 (WT), HSS12 (WT^H1), and HSS13 (WT^H2).

Abbreviation: WT, wild type.