Figure 5.

The OCH1 silencing affects the human PBMC–Sporothrix interaction.

Notes: Yeast-like cells were coincubated with human PBMCs, the supernatant saved, and used to quantify the level of TNFα (A), IL-6 (B), IL-1β (C), and IL-10 (D) by ELISA. Data are represented as mean ± SD obtained with samples from six donors, each assayed in duplicate wells. *P<0.05 when compared to WT cells. The strains used are follows: 1099-18 ATCC MYA 4821 (WT), HSS12 (WT^H1), and HSS13 (WT^H2).

Abbreviations: HK, heat-killed cells; PBMC, peripheral blood mononuclear cell; WT, wild type.