Figure 4. lin-29a neuronal expression is temporally regulated by lin-41 and let-7.

(A) gfp::lin-29a expression in the lin-29a(xe63) strain is precocious in the absence of lin-41. Expression was examined in synchronized L3 animals and compared between lin-41(n2914)/balancer control versus lin-41(n2914) loss-of-function males. Neuronal nuclei are more compact than the larger hypodermal nuclei. (B) gfp::lin-29a expression in the lin-29a(xe63) strain is downregulated in let-7(lf) and lin-41(gf) mutants. Expression was examined at the young adult stage in let-7(n2853ts) loss-of-function mutants and lin-41(xe8) gain-of-function mutants, compared to control males. While control males showed expression of gfp::lin-29a in the hypodermis and nervous system (more compact nuclei), both mutants showed a severe downregulation of gfp::lin-29a expression in the hypodermis and neurons at the young adult stage. L1 let-7(n2853ts) animals were shifted to the restricted temperature (25°C) and imaged as adults, after 48hs.