Table 1.

Impact of a 4-h pulse of low temperature^a on pyronaridine EC₅₀ estimates^b for 3D7 parasites, with and without a drug wash-out.

Median Pyronaridine EC50 (IQR)	Constant 37 °C (n = 5, 5)	18–19 °C 2–7 h post-invasion, then 37 °C (n = 5, 4)c	P valued
48 h continual drug exposure	5.15 nM (1.21–7.87 nM)	3.91 nM (1.17–13.16 nM)	0.50
3–7 h post-invasion, washed out	14.03 nM (1.92–15.51 nM)	32.64 nM (20.95–37.01 nM)	0.065

h: hour.

^aAmbient room temperatures used for the 5 experiments performed were 18, 18, 19, 18 and 19 °C, respectively.

^bA full dose-response curve was generated for each treatment, in five experiments each in technical duplicate.

^cMeaningful data were obtained for only 4 of 5 experiments in the temperature-pulsed, drug pulsed treatment.

^dPaired sign test, one-sided (H_o: 18 °C increases EC₅₀.).