Figure 3. In vivo dynamics of MyD88 KO BM and WT BM progenies in different conditions of recipients. (A) BLI analysis for in vivo dynamics of TCD-BM (non-T cell compartments) cell expansion after transplantation. The migration and expansion of TCD-BM cells were longitudinally monitored at 2 h, days 1, 3, 5, 7, 8 and 9 post-transplantation using an IVIS imaging system 100 (Xenogen, Alameda, CA, USA). (B) At days 5, 7, and 9 post-transplantation, individual target organs, Sp (spleen), Li (liver), Lu (lungs), Ki (kidney), and Int (intestines), were surgically removed from recipient mice and measured for donor-originated infiltrating TCD-BM cells. Representative imaging data are shown. Total body flux (photons/s) values were measured and plotted using Living Image Software (Xenogen) and statistical significance of total body flux at days 5, 7, and 9 was determined. Data (A, B) represent 3 independent experiments.
