Figure 4. Flow cytometric analysis of CD11b⁺Gr-1⁺ cells in progenies in different conditions of BM recipients. (A) Proportion of donor CD11b⁺ and CD11b⁺Gr-1⁺ cells in GVHD and non-GVHD hosts after excluding T cell proportions in spleen on days 5, 7, and 9 post-transplantation. (B) The frequencies and absolute numbers of donor-originated total CD11b⁺ cell and (C) CD11b⁺Gr-1⁺ cells in spleen on days 5, 7, and 9 post-transplantation. (D) Proportion of donor CD11b⁺Gr-1⁺ cells after excluding T cell proportions (CD3^-β2mb⁺) in BM on days 5, 7, and 9 post-transplantation, including intestine, on day 9 post-transplantation. (E) Frequencies and absolute numbers of donor-originated CD11b⁺Gr-1⁺ cells in BM on days 5, 7, and 9 post-transplantation and intestine on day 9 post-transplantation. (F) Representative frequencies of Ly6G^hiLy6C^low or Ly6G^lowLy6C^hi subpopulations within the donor-originated (β2m^b+) CD11b⁺ cells in spleen on days 5, 7, and 9 post-transplantation. (G) Frequencies and absolute numbers of Ly6G^hiLy6C^low or Ly6G^lowLy6C^hi subpopulations within the donor-originated (β2m^b+) CD11b⁺ cells post-transplantation. (H) Apoptosis of donor-derived CD11b⁺Gr-1⁺ cells in GVHD host was analyzed by staining with annexin V Ab on days 5 and 7 post-transplantation. The GMFI values were measured and plotted. Data (A-H) represent more than 3 independent experiments.

^*p<0.05; ^**p<0.01; ^***p<0.001 (WT vs. KO).