Figure 9. MyD88 KO BM-derived DCs isolated from GVHD hosts are able to cross-present Allo-Ag. (A) Ag-presentation assay was performed using WT polyclonal CD8⁺ T cells. CFSE-labeled WT B6 CD8⁺ T cells (CD45.1⁺) were co-cultured with Gr-1⁺ cells (1×10⁵ cells) isolated from spleen of WT and MyD88 KO allogenic GVHD hosts including syngeneic non-GVHD control mice. After co-cultivation for 3 days, the proliferation of polyclonal WT CD8⁺ T cells (CD45.1⁺) was plotted. (B) The CD44 expression on CD8⁺ T cells was measured by flow cytometry. (C) The proportion of DAPI⁺ cells among non-T cell compartments (Gr-1⁺ cells) was plotted. (D) Apoptosis of CD11b⁺CD11c⁺ cells from WT and MyD88-KO BM GVHD (allo), and non-GVHD hosts (syn) was analyzed by following annexin V staining on day 7 post-transplantation. Data (A-D) represent 2 independent experiments.

^*p<0.05 (WT vs. KO).