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. 2018 Nov 12;41(2):765–778. doi: 10.3892/or.2018.6864

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Measurement of total cellular ROS. (A) Flow cytometry analysis: HeLa or SiHa cells with or without 1 h pre-incubation with 5 mM NAC were treated by icaritin for 12 h and a representative result of HeLa cells was shown. (B) Data from three independent experiments in HeLa cells were processed by the GraphPad Prism software (***P<0.001). (C) Time-dependent ROS accumulation. HeLa or SiHa cells were treated by icaritin for 3 h or longer (***P<0.001). (D) Inhibition of icaritin cytotoxicity by NAC. HeLa or SiHa cells with or without pre-incubation (1 h) with 5 mM NAC were treated by icaritin for 48 h and cell survival was analyzed by the MTT assay (*P<0.05; **P<0.01; ***P<0.001). ROS, reactive oxygen species; NAC, N-acetyl cysteine; DCF, dichlorofluorescin; NS, not significant.