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Hypoxia upregulates galectin-3 expression in NSCLC cells. A549 and LK-2 cells were exposed to hypoxia. The (A) mRNA and (B) protein levels of galectin-3 were increased under hypoxic conditions. (C) The levels of galectin-3 released from A549 and LK-2 cells into the culture medium were measured by ELISA. Results are expressed as the means ± SD of three independent experiments. N, normoxic condition (21% O2); H, hypoxic condition (2% O2); NSCLC, non-small cell lung cancer.
