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. 2018 Dec 7;41(2):853–862. doi: 10.3892/or.2018.6915

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Copyright: © Kataoka et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Galectin-3 induces RhoA activation via the upregulation of RhoA expression in the plasma membrane. A549 and LK-2 cells transfected with galectin-3 shRNA (Gal3 shRNA #1) or scrambled control shRNA (scr #1) were cultured under normoxic (N; 21% O₂) or hypoxic (H; 1% O₂) conditions. The levels of RhoA protein, which is related to tumor motility, and vinculin, a cytoskeletal protein, were then measured by western blotting. Levels of RhoA and vinculin expression in (A) the cytoplasm and (B) plasma membrane. β-actin and NaK-ATPase were used as loading controls for the cytoplasm and plasma membrane, respectively. (C) RhoA activity, as measured by ELISA. Wt, wild-type; *P<0.05; **P<0.01; N.S., not significant.