Figure 1.

Alp time- and dose-dependently inhibits the proliferation of LO2 and HepG2 cells. (A) Chemical structure of Alp. (B) Viability analysis of LO2 and HepG2 cells incubated with various concentrations of Alp for 24 h, as assayed using a Cell Counting kit-8 assay. (C) Viability of HepG2 cells incubated with 0, 0.5, 1, 1.5, 2, 5, 10 or 20 µM Alp for 24, 48 or 72 h. The half-maximal inhibitory concentration of Alp for HepG2 cells was determined to be 3.095, 2.333 and 1.681 µM at 24, 48 and 72 h, respectively. Results are presented as the mean ± standard deviation (n=3). Results for each concentration and time point are significantly different (P<0.05) from those for all other concentrations and time points. Alp, alsterpaullone.