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. 2018 Nov 9;41(2):779–788. doi: 10.3892/or.2018.6860

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Copyright: © Lu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Function of CDK5 in different subcellular locations. (A) HGC-27 cells with stably overexpressed CDK5 in different subcellular locations were generated and examined by immunofluorescence. (B and C) The changes in CDK5 expression (overexpression and knockdown) were confirmed using western blotting. (D) CCK-8 and (E) SRB assays were performed to verify the function of CDK5 in regard to cell proliferation. (F) CDK5 overexpression reduced the colony formation of HGC-27 GC cells. (G) CDK5 overexpression reduced the metastasis of HGC-27 GC cells. Magnification, ×200. **P<0.01, as indicated. CDK5, cyclin dependent kinase 5; CCK-8, Cell Counting Kit-8; GC, gastric cancer; EGFP, enhanced green fluorescent protein; WT, wild-type; SRB, Sulforhodamine B; NLS, nuclear localisation signal; NES, nuclear export signal; Nu, nuclear; Cyto, cytoplasm; DOX, doxycycline.