Figure 3.

SIRT6 inhibited the invasion of human osteosarcoma cells. (A) Transwell assay was performed in SAOS-2 cells or (B) MG-63 cells to determine the invasive capacity of cells transfected with SIRT6 construct or vector, respectively. Representative images were shown (magnification, ×20), and data were presented as the mean ± standard deviation, of three independent experiments, **P<0.01 vs. vector. (C) SAOS-2 cells transfected with control siRNA or Si-SIRT6#1, or Si-SIRT6#2 or (D) MG-63 cells. The invasion potential was determined using a Transwell assay. *P<0.05, **P<0.01 vs. NC. SIRT6, sirtuin-6.