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Expression of NF-kB mRNA, MIF, TNF- α and IL-6 in patients with mild, moderate, and severe JOA scores We detected NF-kB mRNA, MIF, and TNF- α in each group by qRT-PCR and ELISA. IL-6 protein, NF-kB, MIF, TNF-α, IL-6 expression levels in all groups increased significantly with the decrease of JOA score. * Statistical difference between the two groups (p<0.05).
