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. 2018 Dec 7;41(2):928–938. doi: 10.3892/or.2018.6921

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Copyright: © Su et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Effect of cytochalasin B (CB) on cell morphology and the F-actin cytoskeleton at different time-points. (A) Optical micrographs. Scale bars, 100 µm. Untreated cells exhibited normal morphology, while the CB-treated cells gradually shrank, rounded up, even became detached from the substrate and floated. (B) In addition, F-actin was disrupted in the treated cells, and the short or punctate actin fragments (green fluorescence) occupied the background of the images. (C) The nucleus (blue fluorescence) appeared irregular at 6 h. (D) Merged images. Scale bars, 50 µm.