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Knockdown of ARHGAP9 by siRNA transfection. ARHGAP9 protein expression was analyzed by (A) western blotting and (B) reverse transcription-quantitative polymerase chain reaction (***P<0.001, ARHGAP9 in MHCC-97L vs. other groups). The efficiency of ARHGAP9 silencing in MHCC-97L cells was measured by (C) reverse transcription-quantitative polymerase chain reaction and (D) western blotting (***P<0.001 vs. siNC). si, small interfering RNA; NC, negative control.
