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. 2018 Nov 15;17(1):965–973. doi: 10.3892/ol.2018.9701

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Copyright: © Sun et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Knockdown of ARHGAP9 attenuates the anti-metastatic effect of Rg3 in MHCC-97L cells. Cells were transfected with siRNA against ARHGAP9 or siNC, and treated with 2.5 and 5 µg/ml Rg3 or DMSO for 24 h. Transwell assays were performed to evaluate (A) cell migration and (B) invasion. ***P<0.001, siRNA vs. siNC; siRNA+2.5µg/ml vs. siNC+2.5µg/ml; siRNA+5µg/ml vs. siNC+5 µg/ml. (C) ARHGAP9 protein expression was measured by western blotting. **P<0.01, siRNA vs. siRNA+2.5 µg/ml; ***P<0.001, siRNA vs. siNC; siRNA+2.5 µg/ml vs. siNC+2.5µg/ml; siRNA+5 µg/ml vs. siNC+5µg/ml; siRNA vs. siRNA+5 µg/ml. si, small interfering RNA; NC, negative control; DMSO, dimethyl sulphoxide.