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. 2018 Oct 17;17(1):127–134. doi: 10.3892/ol.2018.9591

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — The effect of curcumin on the apoptosis of P3X63Ag8 cells. (A) Flow cytometric dot plots of one representative experiment. Results are depicted as logarithmic fluorescence intensity on the x-axis (Annexin V-FITC) vs. y-axis (PI). A total of four quadrants represent necrotic cells (Q1-UL:AV-/PI+), late apoptotic cells (Q1-UR:AV+/PI+), early apoptotic cells (Q1-LR:AV+/PI-) and viable cells (Q1-LL:AV-/PI-). The numbers indicate the percentage of cells in each quadrant and a minimum of 10,000 events were read. (B) The histogram depicts the percentage of early and late apoptosis cells ± standard error of three independent experiments. **P<0.01 vs. the curcumin-untreated group. Cells were treated with 0, 30, 40 and 50 µM curcumin for 24 h. FITC, fluorescein isothiocyanate PI, propidium iodide.