Table I.
Primers used for reverse transcription-quantitative polymerase chain reaction.
| Gene symbol | NCBI Ref seq no. | Sequence (5′→3′) | Product length (bp) |
|---|---|---|---|
| β-actin | NM_007393.4 | 149 | |
| Forward | TGAGAGGGAAATCGTGCGTGAC | ||
| Reverse | GCTCGTTGCCAATAGTGATGACC | ||
| p53 | NM_001127233.1 | 179 | |
| Forward | ACAGGCAGACTTTTCGCCACAG | ||
| Reverse | CCGTCCCAGAAGGTTCCCACT | ||
| Bax | NM_007527.3 | 118 | |
| Forward | TGGAGATGAACTGGACAGCA | ||
| Reverse | GAAGTTGCCATCAGCAAACA | ||
| Bcl-2 | NM_009741.4 | 131 | |
| Forward | CGATTGTGGCAGTCCCTTA | ||
| Reverse | CCAGGATGAAGTGCTCAGGT | ||
| Hes1 | NM_008235.2 | 157 | |
| Forward | CAACACGACACCGGACAAAC | ||
| Reverse | GGAATGCCGGGAGCTATCTT | ||
| Hey1 | NM_010423.2 | 164 | |
| Forward | TGCAGTTAACTCCTCCTTGCC | ||
| Reverse | CGCCGAACTCAAGTTTCCATT |
Bcl-2, B-cell lymphoma 2; Bax, Bcl-2 associated X; Hes1, Hes family BHLH transcription factor 1; Hey1, Hes-related family transcription factor with YRPW motif 1.