Overexpression of miR-34a affects the expression of p53 and Notch-1 in MCF-7 cells that were treated with rhamnetin. (A) Treatment with 25 µM rhamnetin increased p53 expression, and the overexpression of miR-34a and treatment with 25 µM rhamnetin induced this effect further. (B) Treatment with 25 µM rhamnetin decreased Notch1 expression, and the overexpression of miR-34a with 25 µM rhamnetin induced this effect further. (C) Western blots indicating p53 and Notch-1 expression in MCF-7 cells that were treated with rhamnetin and transfected with miR-34a plasmid. **P<0.01 vs. 0 µM rhamnetin; ##P<0.01 vs. 25 µM rhamnetin. 0 µM, control; 25 µM + miR-34a, 25 µM rhamnetin with miR-34a plasmid; miR-34a, microRNA 34a.