Figure 4.
Sporozoite cell invasion assay. Invasion rate on BCEC after treatment for 3 h with methanolic extracts of R. pinnata mature fruits (MeOH Rp) at different concentrations (C1 to C5: 5, 2.5, 1, 0.1, and 0.01 mg/mL). DMSO was used as negative control and as positive controls different concentrations of sulfadoxine/trimethoprim (Veterín-Diftrín 24 Intervet) (C1 to C5: 10/2, 0.1/0.02, 10−3/2 × 10−4, 10−6/2 × 10−7, and 10−9/2 × 10−10 µg/mL) were employed. The invasion rate of treated sporozoites was tested on bovine colonic epithelial cells (BCECs). Statistical significance for MetOH Rp vs. DMSO: P < 0.001 (***) and P < 0.01 (**).