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. 2018 Oct 17;17(1):414–421. doi: 10.3892/ol.2018.9592

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Figure 4. — Overexpression of PTEN enhances the chemosensitivity of OS cells. Cisplatin-resistant MG63 cells transfected with pcDNA3.0-PTEN received Cisplatin treatment and Cisplatin-resistant MG63 cells as control. (A) Relative protein expression of PTEN was compared between the control group and the pcDNA3.0-PTEN group through western blotting. (B) Quantification of the protein expression of PTEN. (C) Proliferation was measured by CCK-8 assay. (D) Cell apoptosis was detected by flow cytometric analysis. The error bars represent means ± standard deviation of three independent experiments. *P<0.05 compared with control group and ^#P<0.05 compared with Cisplatin group. PTEN, phosphatase and tensin homolog; OS, osteosarcoma; CCK-8, Cell Counting Kit-8.