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NCTD inhibits cell viability in osteosarcoma cells. Human osteosarcoma cell lines 143B and SJSA were treated with increasing concentrations of NCTD for 24 or 48 h and cell viability was measured by the CCK8 assay. (A) Chemical structure of NCTD. (B) 143B cells were treated with the indicated doses of NCTD for 24 or 48 h. (C) SJSA cells were treated with the indicated doses of NCTD for 24 or 48 h. Each experiment was repeated in triplicate. *P<0.05 vs. control. NCTD, norcantharidin.
