Table I.

shRNA design sequences.

Gene	Sequence (5′-3′)
NANOG shRNA-1	5′-AACCCTGGAACAGTCCCTTCTATATTCAAGAGATATAGAAGGGACTGTTCCAGGTTTTTTC-3′
	5′-TCGAGAAAAAACCTGGAACAGTCCCTTCTATATCTCTTGAATATAGAAGGGACTGTTCCAGGGTT-3′
NANOG shRNA-2	5′-AACGGGTTAAGCTGTAACATACTTTTCAAGAGAAAGTATGTTACAGCTTAACCCTTTTTTC-3′
	5′-TCGAGAAAAAAGGGTTAAGCTGTAACATACTTTCTCTTGAAAAGTATGTTACAGCTTAACCCGTT-3′
Control shRNA	5′-AACTTCTCCGAACGTGTCACGTTTCAAGAGAACGTGACACGTTCGGAGAATTTTTTC-3′
	5′-TCGAGAAAAAATTCTCCGAACGTGTCACGTTCTCTTGAAACGTGACACGTTCGGAGAAGTT-3′
DBC1 shRNA	5′-AACCCCATCTGTGACTTCCTAGAATTCAAGAGATTCTAGGAAGTCACAGATGGGTTTTTC-3′
	5′-TCGAGAAAAAACCCATCTGTGACTTCCTAGAATCTCTTGAATTCTAGGAAGTCACAGATGGGTT-3′

NANOG, nanoghomeobox; DBC1, deleted in breast cancer 1; sh, short hairpin.