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. 2018 Oct 30;17(1):660–667. doi: 10.3892/ol.2018.9641

Figure 2.

Figure 2.

Hyperacetylation of GRP78 and GRP94 promoter regions is not induced by TSA. (A) Semi-quantitative PCR of WT HCT116 cells using H3K9Ac antibody or IgG. PCR production of GRP78, GRP94 and GAPDH promoter regions was not altered but the positive control p21 promoter region increased following TSA treatment. (B) Quantification results for PCR production of p21, GRP78 and GRP94 promoter regions using H3K9Ac antibody. Quantitative values obtained were normalized relative to the GAPDH promoter. **P<0.01. TSA, trichostatin A; WT, wild type; PCR, polymerase chain reaction; N, negative control; IgG, immunoglobulin G; Ac, acetylation.