Figure 3.

E2F2 is the target of miR-218 in human osteosarcoma cells. (A) Putative binding sites of miR-218 within the E2F2 3′UTR, as predicted using TargetScan and Pictar algorithm software. Bold letters represent the site of action. (B) Expression of E2F2 was verified using western blotting of protein lysates from the stable cell lines. (C) miR-218 decreased luciferase levels in MG63 cells expressing WT E2F2 3′UTR compared with the control group; however, luciferase levels increased in MG63 cells expressing MT E2F2 3′UTR compared with the control group. miR, microRNA; UTR, untranslated region; WT, wild-type; MT, mutant. *P<0.05 vs. control group and miR-218 group.