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. 2018 Oct 19;17(1):365–371. doi: 10.3892/ol.2018.9599

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — The gene transcription of DEDD in HL was inhibited by miR-24-3p. (A) TargetScan was applied to predict the target site of miR-24-3p in the DEDD sequence. (B and C) The luciferase reporter gene assays were performed to detect the fluorescence activities of the DEDD 3′UTR in (B) L1236 and (C) L428 cells which were co-transfected with wild-type DEDD 3′UTR or mutational type DEDD 3′UTR and miR-24-3p, respectively. (D) The DEDD mRNA expression in transfected L1236 and L428 cells was determined using RT-qPCR. (E) The DEDD protein expression was evaluated in transfected L1236 and L428 cells by western blotting. **P<0.01, ***P<0.001. HL, Hodgkin's lymphoma; RT-qPCR, reverse transcription-quantitative PCR.