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. 2018 Oct 11;17(1):42–54. doi: 10.3892/ol.2018.9566

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Copyright: © Zhao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Radiation-induced DNA damage. (A) DNA double-strand breaks were visualized with immunofluorescent staining for γ-H2AX foci. γ-H2AX stained with green fluorescence (fluorescein isothiocyanate) reflects DSBs and merged with nucleus stained with red fluorescence (propidium iodide). Significant dose-dependent increase in foci number was detected following X-irradiation in HeLa cells. Scale bar, 100 µm. (B) Results of western blot analysis demonstrated the expression of γ-H2AX was increased following irradiation. γ-H2AX, phosphorylated histone H2AX.