Figure 5.

Knockdown of MIR31HG inhibited the Wnt/β-catenin signaling pathway in NSCLC cells. (A) The relative protein expression levels of GSK3β, p-GSK3β and β-catenin were detected by western blot analysis following transfection with si-MIR31HG-1 into NCIH460 cells. (B) The relative protein expression levels of GSK3β, p-GSK3β and β-catenin were detected by western blot analysis following transfection with si-MIR31HG-1 into A549 cells. Every independent experiment was performed three times. NSCLC, non-small cell lung cancer; si, small interfering; NC, negative control; p, phosphorylated; GSKβ, glycogen synthase kinase 3β.