Table 1.

Selected examples of experimental protocols for flux measurements in photosynthetic organisms

Organism		Concentrating method (C)	Quenching method (Q)	Extraction method (E)	References
Eukaryote	Chlorella protothecoides	Filtration	− 70 °C MeOH	40:40:20 CH3CN:MeOH:H2O	C	(Wu et al. 2014)
					Q
					E	(Bennett et al. 2008)
	Chlamydomonas reinhardtii *	Centrifugation	− 70 °C MeOH	Mechanical cell disruption with − 70 °C MeOH	C	(Boyle et al. 2017)
					Q
					E
	Phaeodactylum tricornutum *	Centrifugation	Liquid nitrogen	Lyophilization followed by hydrolysis with HCl	C	(Zheng 2013)
					Q
					E
Prokaryote	Synechocystis sp. PCC 6803	Centrifugation	− 40 °C 60% MeOH	50% MeOH	C	(Young et al. Young et al. 2011a, b)
					Q
					E
	Synechococcus elongatus PCC 7942	Centrifugation	0 °C PBS	8:4:3 CHCl3:MeOH:H2O	C	(Jazmin et al. 2017)
					Q
					E	(Folch et al. 1957)
		Centrifugation	− 5 °C minimal BG-11 medium	CHCl3:MeOH	C	(Abernathy et al. 2017)
					Q
					E	(Ma et al. 2014)
	Synechococcus elongatus UTEX 2973
	Synechococcus sp. PCC 7002	Centrifugation	− 20 °C 30% MeOH	− 20 °C 80% MeOH	C	(Qian et al. 2018)
					Q
					E
		Centrifugation	− 20 °C 60% MeOH	− 20 °C 80% MeOH	C	(Qian et al. 2017)
					Q
					E	(Bennette et al. 2011)
		Filtration	− 20 °C 80% MeOH	− 20 °C 80% MeOH	C	(Bennette et al. 2011)
					Q
					E

MeOH Methanol, PBS phosphate buffered saline

*Heterotrophic experiment