Figure 1.

SseL inhibits RPS3 nuclear translocation. HEK293 cells were transfected with the indicated plasmids and treated with TNF-α (50 ng/mL, 30 min) 24 h later. The cells were lysed, separated into nuclear and cytosolic extracts, and subjected to immunoblotting using the indicated antibodies. Poly(ADP-ribose) polymerase (PARP) and tubulin were used to normalize nuclear and cytosolic protein concentrations, respectively. RPS3 quantification data (n = 3) are shown as means ± S.E after normalization to nuclear PARP abundance. Asterisks indicate significantly different protein abundance as compared with the TNF-α control (p < 0.05, Dunn’s multiple comparisons test). Representative images from three independent experiments are shown. We note a small difference in PARP mobility between untreated samples and samples that were either transfected or treated with TNF-α.