Figure 1.

Assessment of CD4⁺ T cells polarization. Mice were immunized once every three weeks. One week after the third vaccination the mice were bled to obtain sera for cytokine analysis. To evaluate cytokine production, TNFα, IFNγ (Th1 and CTL), and IL-4 (Th2) were measured by multiplex assays MHSTCMAG-70K kit. Also, for experimental control, standard curves were made of each cytokine and compared with the expected concentration provided by the kit, as seen in the figure. The results were analyzed using GraphPad Prism software applied to assess the means of the groups by unpaired t-tests. Note: *** p-value < 0.0001; ** p-value < 0.001; * p-value < 0.01. MFI = Median Fluorescence Intensity.