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Assessment of IgG subtype in BALB/c mice. The antibody subclasses were tested on day 59 of the immunized mice. VLP-conjugate vaccines induced the more protective isotypes IgG2a and IgG2b compared to TRAP formulated in alum. In other hand, the group of mice vaccinated with TRAP + alum induced IgG1 subtype, as expected. Note: *** p-value < 0.0001; * p-value < 0.01.
