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. 2018 Dec 10;6(4):70. doi: 10.3390/dj6040070

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of recombinant DSP/PP₂₄₀ proteins on DSP-PP mRNA expression in M2H4 cells. Ninety-percent confluent M2H4 cells in T-25 flasks were treated for eight days with: (1) sf9 conditioned medium (CM); (2) conditioned medium with ascorbic acid (50 μg/mL) (CM + AA); and (3) recombinant DSP/PP₂₄₀ proteins with ascorbic acid (50 μg/mL) (DSP/PP + AA).Cell medium plus treatment was changed every other day.(A) Polymerase chain reaction analyses of DSP-PP and G3PDH mRNA expression. (B) Bar graph of normalized DSP-PP mRNA expression under three different treatments. “1” represents CM, “2” represents CM + AA, and “3” represents DSP/PP + CM. Error bars represent S.E. (n = 3).