Table 1.
Sequence of phosphorylation sites identified in the mouse α4 and β2 nAChR subunits.
| Site | Sequence 1 | Observed Previously 2 | Conserved in Human | Predicted CaMKII Site 3 |
|---|---|---|---|---|
| T417 | …RMDTAVE… | No | No | Yes |
| S444 | …EKASP… | No | Yes (S441) | No |
| S448 | …PSPG… | No | Yes (S445) | No |
| S468 | …KARSLSVQH… | No | Yes (S464) | No |
| S470 | …KARSLSVQH… | Yes | Yes (S467) | Yes |
| S491 | …RSRSIQ… | Yes | Yes (S488) | Yes |
| S521 | …TRPSQLP… | No | No | No |
| S530 | …DQTSPC… | Yes | Yes (S527) | No |
| S540 | …KEPSPVSP… | Yes | Yes (S538) | Yes |
| S543 | …KEPSPVSP… | Yes | Yes (S541) | No |
| S563 | …LPLSPAL… | Yes | Yes (S561) | No |
1 Phosphorylated residue is underlined. 2 Sites of phosphorylation in the α4 nAChR subunit identified in 7, 11, 12, 28–30. 3 CaMKII sites predicted using the Phyre2 site: http://www.sbg.bio.ic.ac.uk/phyre2/.