Figure 2.

Quantitation of spinophilin complexes isolated from dMSNs and iMSNs using tandem mass tag (TMT) analysis. (A) Striatal lysates isolated from male or female mice expressing HA spinophilin under the control of D1 or A2A promoters and treated with saline or amphetamine were immunoprecipitated with an HA antibody, digested with trypsin, labelled with eight different TMT tags, mixed and analyzed by mass spectrometry (MS/MS). (B) A higher intensity of TMT reporter abundance matching spinophilin was observed in amphetamine-treated compared to saline treated animals (t-test; * p < 0.05). (C) Principal component analysis of individual samples normalized to total peptide amount within each sample. (D) A volcano plot showing a majority of the protein have increased abundance in HA immunoprecipitates isolated from amphetamine treated animals.