Table 3.
Spinophilin interacting proteins that had altered abundance ratios in D1 Cre animals compared to A2A Cre animals.
| Description | PSMs | Female A2A Ratios | Female D1 Ratios | Male D1 Avg Ratios | Avg D1/A2A Ratios |
|---|---|---|---|---|---|
| Endophilin-A2 | 12 | 0.50 | 1.38 | 1.28 | 2.63 |
| Cofilin-1 | 14 | 1.13 | 4.14 | 1.57 | 2.52 |
| Malate dehydrogenase, mitochondrial | 27 | 0.92 | 3.22 | 1.38 | 2.51 |
| Calreticulin | 8 | 0.87 | 3.15 | 1.11 | 2.46 |
| Alpha-synuclein | 21 | 1.06 | 3.56 | 1.61 | 2.43 |
| Malate dehydrogenase, cytoplasmic | 41 | 1.05 | 3.59 | 1.38 | 2.36 |
| Glutamate dehydrogenase 1, mitochondrial | 23 | 1.05 | 3.58 | 1.39 | 2.36 |
| Fructose-bisphosphate aldolase | 87 | 1.14 | 3.93 | 1.41 | 2.35 |
| Protein disulfide-isomerase A3 | 9 | 0.93 | 3.19 | 1.13 | 2.31 |
| Citrate synthase, mitochondrial | 32 | 1.37 | 4.58 | 1.55 | 2.24 |
| Synapsin-1 | 42 | 1.11 | 3.51 | 1.45 | 2.23 |
| Tubulin polymerization-promoting protein | 9 | 1.08 | 3.24 | 1.56 | 2.23 |
| Nucleoside diphosphate kinase | 12 | 1.20 | 3.92 | 1.39 | 2.21 |
| Fascin | 26 | 0.89 | 2.78 | 1.13 | 2.20 |
| Cytochrome b-c1 complex subunit 1, mitochondrial | 19 | 0.99 | 3.09 | 1.26 | 2.19 |
| Carbonic anhydrase 2 | 14 | 1.22 | 3.75 | 1.54 | 2.18 |
| Rab GDP dissociation inhibitor alpha | 22 | 1.12 | 3.58 | 1.26 | 2.16 |
| Protein kinase C and casein kinase substrate in neurons protein 1 | 18 | 1.10 | 3.36 | 1.30 | 2.12 |
| Endophilin-A1 | 23 | 1.11 | 3.37 | 1.33 | 2.12 |
| Cytochrome c oxidase subunit 5B, mitochondrial | 13 | 1.17 | 3.52 | 1.43 | 2.12 |
| Adenylyl cyclase-associated protein 2 | 8 | 1.43 | 4.36 | 1.63 | 2.09 |
| Cytochrome b-c1 complex subunit 2, mitochondrial | 40 | 1.04 | 3.08 | 1.22 | 2.06 |
| Pyruvate kinase PKM | 36 | 1.10 | 3.22 | 1.30 | 2.06 |
| Profilin-2 | 14 | 1.33 | 3.85 | 1.58 | 2.04 |
| Myelin proteolipid protein | 38 | 1.58 | 4.56 | 1.89 | 2.04 |
| 60S ribosomal protein L17 | 8 | 1.06 | 3.20 | 1.12 | 2.03 |
| 40S ribosomal protein S23 | 11 | 0.89 | 2.40 | 1.23 | 2.03 |
| Beta-synuclein | 16 | 1.18 | 3.31 | 1.47 | 2.03 |
| Heat shock 70 kDa protein 4 | 23 | 1.07 | 3.01 | 1.27 | 2.01 |
| L-lactate dehydrogenase B chain | 28 | 1.23 | 3.58 | 1.35 | 2.00 |
HA spinophilin was immunoprecipitated from saline and amphetamine-treated D1 Cre and A2A Cre mice. The abundance of the individual proteins was normalized to the abundance of spinophilin. A ratio of the abundance of proteins isolated from the amphetamine treated over the saline treated mice was generated. A second ratio comparing the amphetamine/saline ratios identified in the 3 D1 samples and the 1 A2A sample was generated. Those D1/A2A ratios ≥ 2.00 are shown. A complete list of interacting proteins (without contaminants) and the cell-specific abundance ratios are shown in Table S2B.