Table 4.
Protein biomarkers predictive of treatment response in colorectal cancer obtained by proteomics from colorectal cancer cell lines or animal models.
| CRC Cell Line | Proteomic Approach | Study Focus | Identified Candidate Biomarkers | Reference |
|---|---|---|---|---|
| HCT-116 | iTRAQ, ICAT; LC MALDI-TOF/TOF MS | Butyrate response | Heat shock protein HSP 90-β (HSP90AB1) [P08238] *, galectin-1 (LGALS1) [P09382] *, A-kinase anchor protein 12 (AKAP12) [Q02952] *, vesicle-trafficking protein SEC22b (SEC22B) [O75396] *, cytochrome c oxidase 6b1 (COX6B1) [P14854] * | [71] |
| SW620 | LC MALDI-Q-TOF MS/MS | Irinotecan resistance | α-enolase (ENO1) [P06733], cofilin (CFL1) [P23528], peroxiredoxin-2 (PRDX2) [P32119] * | [64] |
| Colonospheres derived from liver metastases | LC-MS/MS | Cisplatin and oxaliplatin resistance | Baculoviral IAP repeat-containing protein 6 (BIRC6) [Q9NR09] * | [72] |
| DLD-1 | 2-DIGE; LC MALDI-TOF/TOF MS | 5-FU resistance | Heat shock protein beta-1 (HSPB1) [P04792] *, proteasome subunit α type-5 (PSMA5) [P28066], transitional endoplasmic reticulum, ATPase (VCP) [P55072] *, 14-3-3 protein β (YWHAB) [P31946], 14-3-3 protein γ (YWHAG) [P61981], 14-3-3 protein σ (SFN) [P31947], phosphoglycerate kinase 1 (PGK1) [P00558] | [65] |
| GEO | 2-DIGE; LC-MS | Cetuximab resistance | Glucose-6-phosphate 1-dehydrogenase (G6PD) [P11413] *, L-lactate dehydrogenase B chain (LDHB) [P07195], pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (PDHA1) [P08559], transketolase (TKT) [P29401] | [66] |
| HCT-116 | LC-MS/MS | Dasatinib (Src-selective inhibitor) resistance | pY313-protein kinase C delta type (PRKCD) [Q05655] * | [67] |
iTRAQ: Isobaric tag for relative and absolute quantitation; ICAT: Isotope-coded affinity tag; LC-MS/MS: Liquid chromatography tandem-mass spectrometry; 5-FU: 5-fluorouracil; LC-MS: Liquid chromatography-mass spectrometry; MALDI-TOF MS: Matrix assisted laser desorption ionization-time of flight mass spectrometry; MALDI-Q-TOF: MALDI-quadrupole-time of flight; 2D-DIGE: Two-dimensional difference gel electrophoresis. (*) Proteins identified in Zhang’s proteogenomic study [29].