Figure 2.

The C. reinhardtii transcriptome encodes multiple proteins that resemble classical neuropeptide precursors. (A) The strategies used to identify prohormone convertase (PC) and furin cleavage sites, and the amidated products that could be produced from signal peptide-containing soluble C. reinhardtii proteins, are outlined. Potential amidation sites that do not require the action of an endoprotease were also identified. The C. reinhardtii transcriptome was also analyzed using Neuropred (Known Motifs) [5] to identify potential amidated peptides and their lengths. (B) Diagrams illustrate the location of paired basic cleavage sites, furin sites, and amidation sites in three C. reinhardtii proteins that resemble classical neuropeptide precursors.